Detection of Green Fluorescent Protein (eGFP) Tecan Ultra Evolution, Safire and GENios Pro

Green fluorescent protein, GFP, is a spontaneously fluorescent protein isolated from coelenterates, such as the Pacific jellyfish, Aequorea victoria, or from the sea pansy, Renilla reniformis. Its role is to transduce the blue chemiluminescence of aequorin, into green fluorescent light by energy transfer. The molecular cloning of GFP cDNA and the expression of GFP as a functional transgene has opened exciting new avenues of investigation in cell, developmental and molecular biology. GFP can function as a protein tag, as it tolerates Nand C-terminal fusion to a broad variety of proteins many of which have been shown to retain native function. Highly specific intracellular localization including the nucleus, mitochondria, secretory pathway, plasma membrane and cytoskeleton can be achieved via fusion both to whole proteins and individual targeting sequences. The enormous flexibility as a non-invasive marker in living cells allows for numerous other applications such as a cell lineage tracer, reporter of gene expression and as a potential measure of protein-protein interactions.